Using next-generation sequencing to improve DNA barcoding: lessons from a small-scale study of wild bee species (Hymenoptera, Halictidae)
| dc.contributor.author | Sonet, G. | |
| dc.contributor.author | Pauly, A. | |
| dc.contributor.author | Nagy, ZT. | |
| dc.contributor.author | Virgilio, M. | |
| dc.contributor.author | Jordaens, K. | |
| dc.contributor.author | Van Houdt, J. | |
| dc.contributor.author | Worms, S. | |
| dc.contributor.author | De Meyer, M. | |
| dc.contributor.author | Backeljau, T. | |
| dc.date | 2018 | |
| dc.date.accessioned | 2024-03-14T13:15:42Z | |
| dc.date.available | 2024-03-14T13:15:42Z | |
| dc.identifier.uri | https://orfeo.belnet.be/handle/internal/12057 | |
| dc.description | The parallel sequencing of targeted amplicons is a scalable application of next-generation sequencing (NGS) that can advantageously replace Sanger sequencing in certain DNA barcoding studies. It can be used to sequence different PCR products simultaneously, including co-amplified products. Here, we explore this approach by simultaneously sequencing five markers (including the DNA barcode and a diagnostic marker of Wolbachia) in 12 species of Halictidae that were previously DNA barcoded using Sanger sequencing. Consensus sequences were obtained from fresh bees with success rates of 74 100% depending on the DNA fragment. They improved the phylogeny of the group, detected Wolbachia infections (in 8/21 specimens) and characterised haplotype variants. Sequencing cost per marker and per specimen (11.43 ) was estimated to decrease (< 5.00 ) in studies aiming for a higher throughput. We provide guidelines for selecting NGS or Sanger sequencing depending on the goals of future studies. | |
| dc.language | eng | |
| dc.title | Using next-generation sequencing to improve DNA barcoding: lessons from a small-scale study of wild bee species (<EM>Hymenoptera</EM>, <EM>Halictidae</EM>) | |
| dc.type | Article | |
| dc.subject.frascati | Biological sciences | |
| dc.audience | Scientific | |
| dc.subject.free | Invertebrates | |
| dc.source.title | Apidologie | |
| Orfeo.peerreviewed | Yes | |
| dc.identifier.doi | https://doi.org/10.1007/s13592-018-0594-y | |
| dc.identifier.url | https://link.springer.com/article/10.1007/s13592-018-0594-y#citeas | |
| dc.identifier.rmca | 5395 |
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