Towards a better understanding of the snail-trematode-microbiome interactions driving schistosome transmission in Senegal
| dc.contributor.author | Schols, R. | |
| dc.contributor.author | Hammoud, C. | |
| dc.contributor.author | Put, R. | |
| dc.contributor.author | Piot, A. | |
| dc.contributor.author | Maes, T. | |
| dc.contributor.author | Senghor, B. | |
| dc.contributor.author | Decaestecker, E. | |
| dc.contributor.author | Huyse, T. | |
| dc.coverage.spatial | Senegal | |
| dc.date | 2022 | |
| dc.date.accessioned | 2024-03-14T13:26:14Z | |
| dc.date.available | 2024-03-14T13:26:14Z | |
| dc.identifier.uri | https://orfeo.belnet.be/handle/internal/12922 | |
| dc.description |   Background and aims Trematode parasites generally use freshwater snails as obligate intermediate hosts in their lifecycle. A single snail species often serves as an intermediate host for several co-occurring trematode species. This can lead to within-host interactions that can impact infection outcome and subsequent transmission to the final host. Additionally, recent research has emphasized the importance of the snail microbiome in susceptibility to trematode infections. Therefore, it is paramount to document microbiome and trematode communities within individual snail species from natural settings. We focus on a highly endemic setting for schistosomiasis in the Senegal River Basin (SRB). Following the embankment of the Senegal River in the 1980s, a major outbreak of intestinal schistosomiasis occurred. Yet, nowadays, the urinary form is highly prevalent, a shift that remains hitherto unexplained. This study aims to characterize the microbiome and trematode communities of Biomphalaria and Bulinus snail species sampled along the SRB to assess their role in the susceptibility towards schistosome infection.   Methods Snails collected from five localities across the SRB in 2012, 2014 and 2021 are studied. First, a rapid diagnostic PCR is applied to detect infected snails. Second, an amplicon sequencing workflow is used to genotype 10 infected snails per species per site and their infecting trematodes. Finally, 16S metabarcoding is applied to map the microbiome of five non-, five single- and five co-infected snails per species per site.   Results Patterns of genetic diversity, microbiome, and trematode communities of snail hosts in the SRB will be presented and discussed.   Conclusions This will provide greater insight into the processes that influence disease dynamics. | |
| dc.language | eng | |
| dc.title | Towards a better understanding of the snail-trematode-microbiome interactions driving schistosome transmission in Senegal | |
| dc.type | Conference | |
| dc.subject.frascati | Biological sciences | |
| dc.audience | Scientific | |
| dc.subject.free | Invertebrates | |
| dc.source.title | BENELUX CONGRESS OF ZOOLOGY 2022 | |
| Orfeo.peerreviewed | No | |
| dc.identifier.rmca | 6403 |