A scanning electron microscope technique for studying the sclerites of Cichlidogyrus
| dc.contributor.author | Fannes, W. | |
| dc.contributor.author | Vanhove, M. | |
| dc.contributor.author | Huyse, T. | |
| dc.contributor.author | Paladini, G. | |
| dc.date | 2015 | |
| dc.date.accessioned | 2016-03-15T10:07:35Z | |
| dc.date.available | 2016-03-15T10:07:35Z | |
| dc.identifier.uri | https://orfeo.belnet.be/handle/internal/2426 | |
| dc.description | The genus <EM>Cichlidogyrus</EM> (Monogenea: Ancyrocephalidae) includes more than 90 species, most of which are gill parasites of African cichlid fishes. <EM>Cichlidogyrus</EM> has been studied extensively in recent years, but scanning electron microscope (SEM) investigations of the isolated hard parts have not yet been undertaken. In this paper, we describe a method for isolating and scanning the sclerites of individual <EM>Cichlidogyrus</EM> worms. Twenty-year old, formol-fixed specimens of <EM>Cichlidogyrus casuarinus</EM> were subjected to proteinase K digestion in order to release the sclerites from the surrounding soft tissues. SEM micrographs of the haptoral sclerites and the male copulatory organ are presented. The ability to digest formol-fixed specimens makes this method a useful tool for the study of historical museum collections. | |
| dc.language | eng | |
| dc.title | A scanning electron microscope technique for studying the sclerites of <em>Cichlidogyrus</em> | |
| dc.type | Article | |
| dc.subject.frascati | Biological sciences | |
| dc.audience | Scientific | |
| dc.subject.free | Invertebrates | |
| dc.source.title | Parasitology Research | |
| dc.source.volume | 114 | |
| dc.source.page | 2031 2034 | |
| Orfeo.peerreviewed | Yes | |
| dc.identifier.doi | 10.1007/s00436-015-4446-7 | |
| dc.identifier.rmca | 4264 |
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