We have attempted to discriminate between tropospheric and stratospheric burdens of ozone making up the total columns routinely derived above the Jungfraujoch from the spectral analysis of isolated lines. For that, we have identified a number of spectral intervals containing multiple ozone transitions in which the central core of the line and the throughs between these are predominantly sensitive to, respectively, the stratospheric and lower tropospheric contents of ozone. A realistic initial volume mixing ratio profile of ozone is progressively adjusted over various layers until the root square sum of the residuals of the fit to the observations reaches a minimum. The role of the observational geometry in improving the tropospheric/stratospheric O3 ratio is also being investigated.