We conducted a simulation of H2SO4 vapor, H2O vapor, and H2SO4–H2O liquid aerosols from 40 to 100 km, using a 1D Venus cloud microphysics model based on the one detailed in Imamura & Hashimoto. The cloud distribution obtained is in good agreement with in situ observations by Pioneer Venus and remote-sensing observations from Venus Express (VEx). Case studies were conducted to investigate sensitivities to atmospheric parameters, including eddy diffusion and temperature profiles. We find that efficient eddy transport is important for determining upper haze population and its microphysical properties. Using the recently updated eddy diffusion coefficient profile by Mahieux et al., our model replicates the observed upper haze distribution. The H2O vapor distribution is highly sensitive to the eddy diffusion coefficient in the 60–70 km region. This indicates that updating the eddy diffusion coefficient is crucial for understanding the H2O vapor transport through the cloud layer. The H2SO4 vapor abundance varies by several orders of magnitude above 85 km, depending on the temperature profile. However, its maximum value aligns well with observational upper limits found by Sandor et al., pointing to potential sources other than H2SO4 aerosols in the upper haze layer that contribute to the SO2 inversion layer. The best-fit eddy diffusion profile is determined to be ∼2 m2 s−1 between 60 and 70 km and ∼360 m2 s−1 above 85 km. Furthermore, the observed increase of H2O vapor concentration above 85 km is reproduced by using the temperature profile from the VEx/SOIR instrument.