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    An integrated diagnostic setup for the morphological and molecular identification of the Ceratitis FAR complex (C. anonae, C. fasciventris, C. rosa, C. quilicii, Diptera, Tephritidae)

    Authors
    Virgilio, M.
    Daneel, JH.
    Manrakhan, A.
    Delatte, H.
    Meganck, K.
    De Meyer, M.
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    Discipline
    Agricultural biotechnology
    Biological sciences
    Subject
    Invertebrates
    Audience
    Scientific
    Date
    2018
    Metadata
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    Description
    The Ceratitis FAR complex (Diptera, Tephritidae) includes four economically important frugivorous flies (C. anonae, C. fasciventris, C. quilicii, C. rosa) whose immature stages and adult females cannot be properly resolved through morphological identification. In order to develop a simplified molecular tool for the identification of two of these species (C. rosa, C. quilicii) we selected a subset of six microsatellite markers out of a panel of 16 loci that were previously developed for the molecular differentiation of the taxa within the complex. These six markers were first tested in silico and then used for the actual genotyping of C. quilicii and C. rosa, resulting in the correct identification of all male reference specimens. Here we propose an integrated morphological and molecular setup for the identification of the four species of the FAR complex. The decision map relies on preliminary DNA barcoding or morphological identification (when possible) to exclude species not belonging to the complex followed by (a) morphological identification of all adult male specimens and female C. anonae, (b) molecular identification via a panel of 16 microsatellite markers for immature stages, damaged vouchers and samples potentially including adult female C. fasciventris / C. quilicii / C. rosa and (c) molecular identification via a reduced panel of six microsatellite markers for samples including only C. quilicii and C. rosa. This simplified diagnostic setup was profitably implemented in the framework of the ERAfrica fruit fly project and will help correctly identifying species within the FAR complex for their early detection and monitoring.
    Citation
    Virgilio, M.; Daneel, JH.; Manrakhan, A.; Delatte, H.; Meganck, K.; De Meyer, M. (2018). An integrated diagnostic setup for the morphological and molecular identification of the Ceratitis FAR complex (C. anonae, C. fasciventris, C. rosa, C. quilicii, Diptera, Tephritidae). , Bulletin of Entomological Research,
    Identifiers
    uri: https://orfeo.belnet.be/handle/internal/12157
    Type
    Article
    Peer-Review
    Yes
    Language
    eng
    Links
    NewsHelpdeskBELSPO OA Policy

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