Introduction: The introduction of invasive alien species (IAS) can lead to detrimental effects on ecosystems, whether introduced accidentally or intentionally. Preventing the establishment and spread of IAS is crucial due to their disruptive nature and difficulty to eradicate. Early detection and accurate identification are essential for effective management. However, morphology-based identifications are not always feasible, especially for cryptic species or trace materials (hair, feces). Thus, BopCo (Barcoding Facility for Organisms and Tissues of Policy Concern) investigated the utility of DNA sequences for identifying species encompassed in the European Regulation on IAS. Methods: BopCo evaluated online genetic reference databases used to identify the 68 species listed. Factsheets were produced for each species, detailing taxonomy, current distribution in Europe, and a discussion on the use of publicly available DNA sequence data for its identification. Recommendations were provided on suitable DNA markers for IAS identification as well. An overview was compiled to assess data coverage, identify encountered issues, and evaluate the reliability of DNA-based identifications. Results: The investigation yielded concise factsheets for each of the 68 species, offering insights into taxonomy, distribution, and the use of DNA sequence data for identification. Recommendations were provided regarding suitable DNA markers for IAS identification. The assessment revealed challenges such as insufficient sequence data and erroneous taxonomic assignments, impacting the reliability of identifications. Conclusions: BopCo's evaluation underscores the potential of DNA sequences for identifying IAS listed in the European Regulation. However, challenges such as limited sequence data and taxonomic errors highlight the need for improved data coverage and accuracy. Recommendations for enhancing the reliability of DNA-based identifications are crucial for effective IAS management.