The red spot barb Barbus kerstenii was first described by from East Africa. It is widespread in Central and East Africa to Lake Victoria basin, Cunene, Okavango, upper Zambezi, tributaries of the lower Zambezi and the Save-Runde Systems. It also occurs in Lake Tanganyika and Lake Kivu drainage basins. The species has been reported synonymously as Barbus lumiensis, B. minchini, B. akeleyi, B. nigrolinea, B. salmo, B. zanzibaricus pauior, B. loveridgii, B. mohasicus, B. rufua, B. serrifer and Labeobarbus kerstenii. There have been concerns that several populations included in this species might represent distinct taxa. This study undertook a morphometric revision of B. kerstenii from East, Central and southern Africa specimens at the RMCA. PCAs were carried on 10 meristics and 18 measurements on the specimens categorized by locality. Pre-pectoral length and body depth at dorsal expressed as percentages of standard length were the most diagnostic morphometric characters. Results showed a clear separation of specimens from Akagera (Nile system), from upper Congo. However, Lake Kivu specimens eclipsed the two groups, appearing intermediary.Akagera specimens compared well with the lectotypes for B. kerstenii, B. loveridgii and B. lumiensis holotype. Lake Kivu upper and Congo specimens corresponded with the syntypes of B. minchini. Specimens from Kenya coastal region clustered together with the holotype of B. loveridgii, from Amala River, Lake Baringo, Kenya. The apparent distinctions in specimens were largely associated to locality.