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    Problematic barcoding in flatworms: A case-study on monogeneans and rhabdocoels (Platyhelminthes)

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    Authors
    Vanhove, M.
    Tessens, B.
    Schoelinck, C.
    Jondelius, U.
    Littlewood, T.
    Artois, T.
    Huyse, T.
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    Discipline
    Biological sciences
    Subject
    Invertebrates
    Audience
    Scientific
    Date
    2013
    Metadata
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    Description
    Some taxonomic groups are less amenable to mitochondrial DNA barcoding than others. Due to the paucity of molecular information of understudied groups and the huge molecular diversity within flatworms, primer design has been hampered. Indeed, all attempts to develop universal flatworm-specific COI markers have failed so far. We demonstrate how high molecular variability and contamination problems limit the possibilities for barcoding using standard COI-based protocols in flatworms. As a consequence, molecular identification methods often rely on other widely applicable markers. In the case of Monogenea, a very diverse group of platyhelminth parasites, and Rhabdocoela, representing one-fourth of all free-living flatworm taxa, this has led to a relatively high availability of nuclear ITS and 18S/28S rDNA sequences on GenBank. In a comparison of the effectiveness in species assignment we conclude that mitochondrial and nuclear ribosomal markers perform equally well. In case intraspecific information is needed, rDNA sequences can guide the selection of the appropriate (i.e. taxon-specific) COI primers if available.
    Citation
    Vanhove, M.; Tessens, B.; Schoelinck, C.; Jondelius, U.; Littlewood, T.; Artois, T.; Huyse, T. (2013). Problematic barcoding in flatworms: A case-study on monogeneans and rhabdocoels (Platyhelminthes). , ZooKeys, Vol. 365; DNA barcoding: a practical tool for fundamental and applied biodiversity research, 355-379, DOI: 10.3897/zookeys.365.5776.
    Identifiers
    uri: https://orfeo.belnet.be/handle/internal/1837
    doi: http://dx.doi.org/10.3897/zookeys.365.5776
    Type
    Article
    Peer-Review
    Yes
    Language
    eng
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