Automating the calculation of the ad hoc distance thresholds for DNA barcoding identification using two R functions
dc.contributor.author | Sonet, G. | |
dc.contributor.author | Jordaens, K. | |
dc.contributor.author | Nagi, ZT. | |
dc.contributor.author | Breman, F. | |
dc.contributor.author | De Meyer, M. | |
dc.contributor.author | Backeljau, T. | |
dc.contributor.author | Virgilio, M. | |
dc.date | 2013 | |
dc.date.accessioned | 2016-03-15T10:06:52Z | |
dc.date.available | 2016-03-15T10:06:52Z | |
dc.identifier.uri | https://orfeo.belnet.be/handle/internal/2021 | |
dc.description | The probability of erroneous DNA barcoding identification is higher when identification is based on a large distance between the query (the barcode sequence of the specimen to identify) and its best match in a reference barcode library. Yet, the number of these incorrect identifications can be reduced by setting a distance threshold above which identification is considered unreliable. To this end, it has been proposed to use an ad hoc distance threshold producing identifications with a user-defined error probability (e.g. 5%). Here, we propose two R scripts to automate the calculation of ad hoc distance thresholds for reference libraries of DNA barcodes. Both scripts, a user manual and an example file are freely available upon request on the JEMU website (http://jemu.myspecies.info). | |
dc.language | eng | |
dc.title | Automating the calculation of the <EM>ad hoc</EM> distance thresholds for DNA barcoding identification using two R functions | |
dc.type | Conference | |
dc.subject.frascati | Biological sciences | |
dc.audience | Scientific | |
dc.subject.free | Invertebrates | |
dc.source.title | 5th International Barcode of Life Conference (Kunming, P.R. China) | |
dc.source.page | 174 | |
Orfeo.peerreviewed | No | |
dc.identifier.rmca | 3375 |
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