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    Automating the calculation of the ad hoc distance thresholds for DNA barcoding identification using two R functions

    Authors
    Sonet, G.
    Jordaens, K.
    Nagi, ZT.
    Breman, F.
    De Meyer, M.
    Backeljau, T.
    Virgilio, M.
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    Discipline
    Biological sciences
    Subject
    Invertebrates
    Audience
    Scientific
    Date
    2013
    Metadata
    Show full item record
    Description
    The probability of erroneous DNA barcoding identification is higher when identification is based on a large distance between the query (the barcode sequence of the specimen to identify) and its best match in a reference barcode library. Yet, the number of these incorrect identifications can be reduced by setting a distance threshold above which identification is considered unreliable. To this end, it has been proposed to use an ad hoc distance threshold producing identifications with a user-defined error probability (e.g. 5%). Here, we propose two R scripts to automate the calculation of ad hoc distance thresholds for reference libraries of DNA barcodes. Both scripts, a user manual and an example file are freely available upon request on the JEMU website (http://jemu.myspecies.info).
    Citation
    Sonet, G.; Jordaens, K.; Nagi, ZT.; Breman, F.; De Meyer, M.; Backeljau, T.; Virgilio, M. (2013). Automating the calculation of the ad hoc distance thresholds for DNA barcoding identification using two R functions. , 5th International Barcode of Life Conference (Kunming, P.R. China), 174,
    Identifiers
    uri: https://orfeo.belnet.be/handle/internal/2021
    Type
    Conference
    Peer-Review
    No
    Language
    eng
    Links
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