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    Identification of Belgian mosquito species (Diptera: Culicidae) by DNA barcoding

    Authors
    V Versteirt,
    ZT Nagy,
    P Roelants,
    L Denis,
    FC Breman,
    N Smitz,
    D Damiens,
    W Dekoninck,
    T Backeljau,
    M Coosemans,
    W Van Bortel,
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    Discipline
    Biological sciences
    Subject
    Invertebrates
    Audience
    Scientific
    Date
    2015
    Metadata
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    Description
    Background Since its introduction in 2003, DNA barcoding has proven to be a promising method for the identification of many taxa, including mosquitoes (Diptera: Culicidae). Many mosquito species are potential vectors of pathogens, and correct identification in all life stages is essential for effective mosquito monitoring and control. To use DNA barcoding for species identification, a reliable and comprehensive reference database of verified DNA sequences is required. Results Genetic diversity of mosquitoes in Belgium was assessed using a 658 bp fragment of the mitochondrial cytochrome oxidase I (COI) gene, and a reference data set was established. Most species appeared as well-supported clusters. Intraspecific Kimura 2-parameter (K2P) distances averaged 0.7%, and the maximum observed K2P distance was 6.2% for Aedes koreicus. A small overlap between intra- and interspecific K2P distances for congeneric sequences was observed. Overall, the identification success using best match and the best close match criteria were high, that is above 98%. No clear genetic division was found between the closely related species Aedes annulipes and Aedes cantans, which can be confused using morphological identification only. The members of the Anopheles maculipennis complex, that is Anopheles maculipennis s.s. and An. messeae, were weakly supported as monophyletic taxa. Significance This study showed that DNA barcoding offers a reliable framework for mosquito species identification in Belgium except for two pairs of closely related species.
    Citation
    V Versteirt,; ZT Nagy,; P Roelants,; L Denis,; FC Breman,; N Smitz,; D Damiens,; W Dekoninck,; T Backeljau,; M Coosemans,; W Van Bortel, (2015). Identification of Belgian mosquito species (Diptera: Culicidae) by DNA barcoding. , 6th International Barcode of Life Conference,
    Identifiers
    uri: https://orfeo.belnet.be/handle/internal/2496
    Type
    Conference
    Peer-Review
    No
    Language
    eng
    Links
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