Overall, the digitalization of herbaria in the Democratic Republic of the Congo (DRC) has already reached a high level. All herbaria in the country have begun to digitize their holdings for long term conservation of information and images on African plants. About 20,000 plant specimens have already been scanned and their associated metadata encoded or updated. Using these basic data, determination gap analyses can be conducted to determine the areas yet to explore. Such gap analysis has been undertaken in the Kivu region using the botanical collections preserved in Lwiro (LWI) and Mulungu (MLGU) in order to orient future botanical inventories. The gap analysis here presented covers three provinces: North-Kivu, South-Kivu and Maniema. Specimens from LWI and MLGU were studied taxonomically and the specimens were georeferenced using the index of collecting localities detailed by Bamps (1982). The areas of the future botanical inventories in the Kivu have been identified using the Geographical Information System software DivaGIS (http://www.diva-gis.org/). An analysis of specimens previously collected in the eastern part of Kivu in the Democratic Republic of Congo has been done via the collection management software Botanical Research And Herbarium Management System, BRAHMS (http://herbaria.plants.ox.ac.uk/bol/brahms/), which is in use at the herbaria of Lwiro and Mulungu. This has enabled identification of four large areas (Kahuzi-Biega National Park at low altitude, Itombwe Reserve, Virunga National Park and Maiko National Park) that are good candidates for the upcoming inventories.